Low paraoxonase in Persian Gulf War Veterans self-reporting Gulf War Syndrome

被引:51
作者
Mackness, B [1 ]
Durrington, PN [1 ]
Mackness, MI [1 ]
机构
[1] Univ Manchester, Manchester Royal Infirm, Dept Med, Manchester M13 9WL, Lancs, England
关键词
paraoxonase; Gulf War; Gulf War Syndrome;
D O I
10.1006/bbrc.2000.3526
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Exposure to organophosphate (OP's) insecticides and nerve gases during the Persian Gulf War has keen implicated in the development of Gulf War Syndrome. Paraoxonase (PON1) present in human serum detoxifies OP's. We determined the levels of PON1 in the serum of Gulf War Veterans and compared these to those found in a control population. One hundred fifty-two Gulf War Veterans from the UK who self-reported the presence of Gulf War Syndrome via a questionnaire and 152 age and gender matched controls were studied. PON1 activity, concentration, and genotype were determined. In the Gulf War Veterans, paraoxon hydrolysis was less than 50% of that found in the controls (100.3 (14.8-233.8) vs 214.6 (50.3-516.2) nmol/min/ml, P < 0.001). This low activity was independent of the effect of PON1 genotype. The serum PON1 concentration was also lower in the Gulf War Veterans (75.7 (18.1-351.3) vs 88.2 (34.5-527.4) mu g/ml, P < 0.00025), which was again independent of PON1 genotype. There was no difference in the rate of diazoxon hydrolysis between the groups (10.2 +/- 4.1 mu mol/min/ml vs 9.86 +/- 4.4, P = NS). A decreased capacity to detoxify OP insecticides resulting from low serum PON1 activity may have contributed to the development of Gulf War Syndrome. (C) 2000 Academic Press.
引用
收藏
页码:729 / 733
页数:5
相关论文
共 26 条
[1]   RELATIONSHIP BETWEEN SERUM BUTYRYLCHOLINESTERASE ACTIVITY, HYPERTRIGLYCERIDEMIA AND INSULIN SENSITIVITY IN DIABETES-MELLITUS [J].
ABBOTT, CA ;
MACKNESS, MI ;
KUMAR, S ;
OLUKOGA, AO ;
GORDON, C ;
ARROL, S ;
BHATNAGAR, D ;
BOULTON, AJM ;
DURRINGTON, PN .
CLINICAL SCIENCE, 1993, 85 (01) :77-81
[2]  
ADKINS S, 1993, AM J HUM GENET, V52, P598
[3]   Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities - Selective action of human paraoxonase allozymes Q and R [J].
Aviram, M ;
Billecke, S ;
Sorenson, R ;
Bisgaier, C ;
Newton, R ;
Rosenblat, M ;
Erogul, J ;
Hsu, C ;
Dunlop, C ;
La Du, B .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1998, 18 (10) :1617-1624
[4]   Serum paraoxonase after myocardial infarction [J].
Ayub, A ;
Mackness, MI ;
Arrol, S ;
Mackness, B ;
Patel, J ;
Durrington, PN .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1999, 19 (02) :330-335
[5]   IDENTIFICATION OF A DISTINCT HUMAN HIGH-DENSITY-LIPOPROTEIN SUBSPECIES DEFINED BY A LIPOPROTEIN-ASSOCIATED PROTEIN, K-45 - IDENTITY OF K-45 WITH PARAOXONASE [J].
BLATTER, MC ;
JAMES, RW ;
MESSMER, S ;
BARJA, F ;
POMETTA, D .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 211 (03) :871-879
[6]   The effect of the human serum paraoxonase polymorphism is reversed with diazoxon, soman and sarin [J].
Davies, HG ;
Richter, RJ ;
Keifer, M ;
Broomfield, CA ;
Sowalla, J ;
Furlong, CE .
NATURE GENETICS, 1996, 14 (03) :334-336
[7]   A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].
ELLMAN, GL ;
COURTNEY, KD ;
ANDRES, V ;
FEATHERSTONE, RM .
BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&
[8]   QUANTIFICATION OF HUMAN SERUM PARAOXONASE BY ENZYME-LINKED IMMUNOASSAY - POPULATION DIFFERENCES IN PROTEIN CONCENTRATIONS [J].
GARIN, MCB ;
ABBOTT, C ;
MESSMER, S ;
MACKNESS, M ;
DURRINGTON, P ;
POMETTA, D ;
JAMES, RW .
BIOCHEMICAL JOURNAL, 1994, 304 :549-554
[9]   MODERATE ALCOHOL INTAKE, INCREASED LEVELS OF HIGH-DENSITY-LIPOPROTEIN AND ITS SUBFRACTIONS, AND DECREASED RISK OF MYOCARDIAL-INFARCTION [J].
GAZIANO, JM ;
BURING, JE ;
BRESLOW, JL ;
GOLDHABER, SZ ;
ROSNER, B ;
VANDENBURGH, M ;
WILLETT, W ;
HENNEKENS, CH .
NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (25) :1829-1834
[10]   Self-reported exposure to neurotoxic chemical combinations in the Gulf War - A cross-sectional epidemiologic study [J].
Haley, RW ;
Kurt, TL .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1997, 277 (03) :231-237