Molecular identification and characterization of the tomato flagellin receptor LeFLS2, an orthologue of Arabidopsis FLS2 exhibiting characteristically different perception specificities

被引:156
作者
Robatzek, Silke
Bittel, Pascal
Chinchilla, Delphine
Koechner, Petra
Felix, Georg
Shiu, Shin-Han
Boller, Thomas
机构
[1] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
[2] Univ Basel, Bot Inst, Zurich Bsed Plant Sci Ctr, CH-4056 Basel, Switzerland
[3] Max Planck Inst plant Breeding Res, Dept Plant Microbe Interact, D-72076 Tubingen, Germany
[4] Univ Tubingen, ZMBP, D-72076 Tubingen, Germany
[5] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
关键词
FLS2; homologue; flg22; immunity; PAMP; Solanaceae;
D O I
10.1007/s11103-007-9173-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacterial flagellin is known to stimulate host immune responses in mammals and plants. In Arabidopsis thaliana, the receptor kinase FLS2 mediates flagellin perception through physical interaction with a highly conserved epitope in the N-terminus of flagellin, represented by the peptide flg22 derived from Pseudomonas syringae. The peptide flg22 is highly active as an elicitor in many plant species. In contrast, a shortened version of the same epitope derived from Escherichia coli, flg15(E coli), is highly active as an elicitor in tomato but not in A. thaliana or Nicotiana benthamiana. Here, we make use of these species-specific differences in flagellin perception abilities to identify LeFLS2 as the flagellin receptor in tomato. LeFLS2 is most closely related to AtFLS2, indicating that it may represent the flagellin receptor of tomato. Expression of the LeFLS2 gene in Arabidopsis did not result in accumulation of its corresponding gene product, as indicated by experiments with LeFLS2-GFP fusions. In contrast, expression of LeFLS2-GFP fusions in N. benthamiana, a species that, like tomato, belongs to the Solanaceae, was obviously functional. N. benthamiana plants transiently expressing a LeFLS2-GFP fusion acquired responsiveness to flg15(E coli) to which they are normally unresponsive. Thus, LeFLS2 encodes a functional, specific flagellin receptor, the first to be identified in a plant family other than the Brassicaceae.
引用
收藏
页码:539 / 547
页数:9
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