Methanogens and sulfate-reducing bacteria in oil sands fine tailings waste

被引:118
作者
Holowenko, FM
MacKinnon, MD
Fedorak, PM [1 ]
机构
[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
[2] Syncrude Canada Ltd, Edmonton Res Ctr, Edmonton, AB T6N 1H4, Canada
关键词
fine tailings; methanogens; sulfate-reducing bacteria; methane; oil sands;
D O I
10.1139/cjm-46-10-927
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of methane in the fine tailings zone of this and other settling basins may affect the performance of these settling basins and impact reclamation options. Enumeration studies found methanogens (10(5)-10(6) MPN/g) within the fine tailings zone of various oil sands waste settling basins. SRB were also present (10(4)-10(5) MPN/g) with elevated numbers when sulfate was available. The methanogenic population was robust, and sample storage up to 9 months at 4%C did not cause the MPN values to change. Nor was the ability of the consortium to produce methane delayed or less efficient after storage. Under laboratory conditions, fine tailings samples released 0.10-0.25 mL CH4 (at STP)/mL fine tailings. The addition of sulfate inhibited methanogenesis by stimulating bacterial competition.
引用
收藏
页码:927 / 937
页数:11
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