Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans

被引：42

作者：

Takanami, T ^{[1
]}

Mori, A ^{[1
]}

Takahashi, H ^{[1
]}

Higashitani, A ^{[1
]}

机构：

[1] Tohoku Univ, Inst Genet Ecol, Sendai, Miyagi 9808577, Japan

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 21期

关键词：

D O I：

10.1093/nar/28.21.4232

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sensitivity of meiotic cells to DNA damaging agents is little understood. We have demonstrated that the meiotic-pachytene nuclei in the Caenorhabditis elegans gonad are hyper-resistant to X-ray irradiation, but not to UV irradiation, whereas the early embryonic cells after fertilization and the full grown oocytes are not. The Ce-rdh-1 gene [RAD51, DMC1 (LIM15), homolog 1 or Ce-rad-51], which is essential for the meiotic recombination, is the only bacterial recA-like gene in the nematode genome, and is strongly expressed in the meiotic cells. Following silencing of the Ce-rdh-1 gene by RNA interference, the meiotic cells become more sensitive to X-ray irradiation than the early embryonic cells. This is the first report that meiotic cells are hyper-resistant to DNA strand breaks due to the high level of expression of the enzyme(s) involved in meiotic homologous recombination.

引用

页码：4232 / 4236

页数：5

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