Sublingual immunization with an HIV subunit vaccine induces antibodies and cytotoxic T cells in the mouse female genital tract

被引：53

作者：

Hervouet, Catherine ^{[1
,2
,3
]}

Luci, Carmelo ^{[1
,2
,3
]}

Cuburu, Nicolas ^{[2
,3
]}

Cremel, Magali ^{[2
,3
]}

Bekri, Selma ^{[1
,2
]}

Vimeux, Lene ^{[4
,5
]}

Maranon, Concepcion ^{[4
,5
]}

Czerkinsky, Cecil ^{[3
,6
]}

Hosmalin, Anne ^{[4
,5
]}

Anjuere, Fabienne ^{[1
,2
,3
]}

机构：

[1] Fac Med Pasteur, INSERM, U634, F-06107 Nice 2, France

[2] Univ Nice Sophia Antipolis, F-06107 Nice 2, France

[3] Fac Med Pasteur, INSERM, U721, F-06107 Nice 2, France

[4] Univ Paris 05, CNRS, UMR 8104, Inst Cochin, Paris, France

[5] Fac Med Nice, INSERM, U1016, F-06034 Nice, France

[6] Int Vaccine Inst, Seoul 151818, South Korea

来源：

VACCINE | 2010年 / 28卷 / 34期

关键词：

Sublingual vaccination; Genital immunity; HIV; HUMAN-IMMUNODEFICIENCY-VIRUS; TOXIN B-SUBUNIT; CHOLERA-TOXIN; IMMUNE-RESPONSES; DENDRITIC CELLS; MUCOSAL IMMUNITY; SECRETING CELLS; IN-VIVO; NEUTRALIZING ANTIBODIES; INTRANASAL IMMUNIZATION;

D O I：

10.1016/j.vaccine.2010.06.033

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A vaccine against heterosexual transmission by human immunodeficiency virus (HIV) should generate cytotoxic and antibody responses in the female genital tract and in extra-genital organs. We report that sublingual immunization with HIV-1 gp41 and a reverse transcriptase polypeptide coupled to the cholera toxin B subunit (CTB) induced gp41-specific IgA antibodies and antibody-secreting cells, as well as reverse transcriptase-specific CD8 T cells in the genital mucosa, contrary to intradermal immunization. Conjugation of the reverse transcriptase peptide to CTB favored its cross-presentation by human dendritic cells to a T cell line from an HIV+ patient. Sublingual vaccination could represent a promising vaccine strategy against heterosexual transmission of HIV-1. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：5582 / 5590

页数：9

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