Characterization of Moroccan isolates of Verticillium dahliae Kleb using RAPD markers

被引:10
作者
Cherrab, M [1 ]
Serrhini, MN
Charest, PM
机构
[1] Univ Chouaib Doukkali, Fac Sci, Dept Biol, El Jadida, Morocco
[2] Univ Laval, Dept Phytol, Quebec City, PQ, Canada
[3] Ecole Natl Agr, Dept Phytopathol, Meknes, Morocco
来源
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 2000年 / 148卷 / 04期
关键词
Verticillium dahliae; olive tree (Olea europea var. sativa); Morocco; random amplified polymorphic DNA (RAPD);
D O I
10.1046/j.1439-0434.2000.00500.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Isolates of Verticillium dahliae were sampled from different olive tree orchards in Morocco. These olive trees were located in different commercial culture locations in southern, central and northern Morocco. The isolates were characterized using genetic markers obtained after their DNA PCR amplification with random amplified polymorphic DNA (RAPD) primers. Among the 40 primers tested, 10 generated a total of 66 polymorphic fragments. Among the 38 isolates of V. dahliae tested, RAPD markers were successful in the characterization of groups based on their geographic origin. With the exception of one specific isolate, no correlation could be established among the isolates, based on the morphological appearance of the colony in culture.
引用
收藏
页码:243 / 249
页数:7
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