Detection of exogenous hydrocortisone in horse urine by gas chromatography combustion carbon isotope ratio mass spectrometry

被引：18

作者：

Aguilera, R

Becchi, M

Mateus, L

Popot, MA

Bonnaire, Y

Casabianca, H

Hatton, CK

机构：

[1] CNRS, Serv Cent Anal, F-69390 Vernaison, France

[2] Lab Federat Natl Soc Courses, LAB, F-92290 Chatenay Malabry, France

[3] Univ Calif Los Angeles, Olymp Lab, Dept Mol & Med Pharmacol, Los Angeles, CA 90025 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 702卷 / 1-2期

关键词：

hydrocortisone; corticosteroids;

D O I：

10.1016/S0378-4347(97)00375-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatography-combustion-isotope ratio mass spectrometry method for confirmation of hydrocortisone abuse in horseracing and equine sports is proposed. Urinary hydrocortisone was converted to a bismethylenedioxy derivative which presents good gas chromatographic properties and brings an extra carbon contribution of only two carbon atoms. Synthetic hydrocortisone has a different C-13 abundance from that of natural urinary horse hydrocortisone and the difference is significant, therefore exogenous and endogenous hydrocortisone can be distinguished. (C) 1997 Elsevier Science B.V.

引用

页码：85 / 91

页数：7

共 17 条

[1] Detection of testosterone misuse: Comparison of two chromatographic sample preparation methods for gas chromatographic-combustion/isotope ratio mass spectrometric analysis [J].