In vitro measurement of the frequency-dependent attenuation in cancellous bone between 0.2 and 2 MHz

Our goal was to evaluate the frequency dependence of the ultrasonic attenuation coefficient in cancellous bone. Estimates were obtained in immersion, using a substitution method in the through-transmit mode, by scanning 14 human bone specimens (calcaneus). Measurements were performed with three pairs of focused transducers with a center frequency of 0.5, 1.0, and 2.25 MHz, respectively in order to cover an extended frequency bandwidth (0.2-1.7 MHz). When the experimental attenuation coefficient values were modeled with a nonlinear power fit alpha(f) = alpha(0) + alpha(1)f(n), the attenuation coefficient was found to increase as f(1.09 +/- 0.3) over the measurement bandwidth. However, a substantial variation of the exponent n (0.4-2.2) within specimens and also between specimens was observed. The acoustical parameters were compared to bone mineral density. A highly significant relationship was noted between alpha(1) and BMD (r(2) = 0.75, p < 10(-4)). No correlation was found between n and BMD. Several attenuation mechanisms are discussed as well as the potential impact these results may have in in vivo quantitative measurements. (C) 2000 Acoustical Society of America. [S0001-4966(00)04009-1].