Histone acetyltransferases and deacetylase in Entamoeba histolytica

被引:38
作者
Ramakrishnan, G
Gilchrist, CA
Musa, H
Torok, MS
Grant, PA
Mann, BJ
Petri, WA [1 ]
机构
[1] Univ Virginia, Dept Med, Charlottesville, VA 22908 USA
[2] Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA
[3] Univ Virginia, Dept Pathol, Charlottesville, VA 22908 USA
[4] Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA
关键词
Entamoeba; histone acetyltransferase; histone deacetylase; acetylation; transcription;
D O I
10.1016/j.molbiopara.2004.09.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In our efforts to understand how transcription may be regulated in Entamoeba histolytica, we have examined if this parasite has conserved enzymatic mechanisms for targeted acetylation and deacetylation of histories. Western blotting indicated that basic nuclear proteins in the size range of 16-23 kDa were acetylated in amebic trophozoites, suggesting histone acetylation. Single representatives of the GNAT and MYST family of histone acetyltransferases (HATs) were identified in the E. histolytica genome and their expression in amebic trophozoites was detected by reverse transcription of RNA followed by the polymerase chain reaction (RT-PCR). Full-length recombinant EhMYST protein demonstrated HAT activity with calf thymus histories and showed a preference for histone H4, similar to the yeast MYST protein, Esa1. However, ehMYST did not complement a yeast esa1 mutation. Histone deacetylase (HDAC) activity was detected in nuclear extracts from E. histolytica, and characteristically, was inhibited by trichostatin A (TSA). Consistent with the observation of HDAC activity, RT-PCR analysis demonstrated that an amebic hdac1 homolog (ehHDAC) is expressed and appropriately spliced in E. histolytica trophozoites. Our results suggest that mechanisms for histone acetylation and deacetylation are operational in E. histolytica. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:205 / 216
页数:12
相关论文
共 45 条
[1]   NuRD and SIN3 - histone deacetylase complexes in development [J].
Ahringer, J .
TRENDS IN GENETICS, 2000, 16 (08) :351-356
[2]  
[Anonymous], 1997, Wkly Epidemiol Rec, V72, P97
[3]   SEQUENCE AND ORGANIZATION OF AN UNUSUAL HISTONE H4 GENE IN THE HUMAN PARASITE ENTAMOEBA-HISTOLYTICA [J].
BINDER, M ;
ORTNER, S ;
PLAIMAUER, B ;
FODINGER, M ;
WIEDERMANN, G ;
SCHEINER, O ;
DUCHENE, M .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 71 (02) :243-247
[4]   Acetylation of histone H4 by Esa1 is required for DNA double-strand break repair [J].
Bird, AW ;
Yu, DY ;
Pray-Grant, MG ;
Qiu, QF ;
Harmon, KE ;
Megee, PC ;
Grant, PA ;
Smith, MM ;
Christman, MF .
NATURE, 2002, 419 (6905) :411-415
[5]   The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation [J].
Bruchhaus, I ;
Loftus, BJ ;
Hall, N ;
Tannich, E .
EUKARYOTIC CELL, 2003, 2 (03) :501-509
[6]   Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscess [J].
Bruchhaus, I ;
Roeder, T ;
Lotter, H ;
Schwerdtfeger, M ;
Tannich, E .
MOLECULAR MICROBIOLOGY, 2002, 44 (04) :1063-1072
[7]   UNUSUAL GENE ORGANIZATION IN THE PROTOZOAN PARASITE ENTAMOEBA-HISTOLYTICA [J].
BRUCHHAUS, I ;
LEIPPE, M ;
LIOUTAS, C ;
TANNICH, E .
DNA AND CELL BIOLOGY, 1993, 12 (10) :925-933
[8]   Histone acetyltransferase activity and interaction with ADA2 are critical for GCN5 function in vivo [J].
Candau, R ;
Zhou, JX ;
Allis, CD ;
Berger, SL .
EMBO JOURNAL, 1997, 16 (03) :555-565
[9]   The diverse functions of histone acetyltransferase complexes [J].
Carrozza, MJ ;
Utley, RT ;
Workman, JL ;
Côté, J .
TRENDS IN GENETICS, 2003, 19 (06) :321-329
[10]   Histone deacetylases (HDACs): characterization of the classical HDAC family [J].
De Ruijter, AJM ;
Van Gennip, AH ;
Caron, HN ;
Kemp, S ;
Van Kuilenburg, ABP .
BIOCHEMICAL JOURNAL, 2003, 370 :737-749