Optimization of cyclomaltodextrin glucanotransferase production from Bacillus firmus

被引：44

作者：

Gawande, BN ^{[1
]}

Singh, RK ^{[1
]}

Chauhan, AK ^{[1
]}

Goel, A ^{[1
]}

Patkar, AY ^{[1
]}

机构：

[1] Natl Chem Lab, Div Chem Engn, Pune 411008, Maharashtra, India

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1998年 / 22卷 / 04期

关键词：

cyclomaltodextrin glucanotransferase; Bacillus firmus; factorial design; media optimization;

D O I：

10.1016/S0141-0229(97)00184-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Production of cyclomaltodextrin glucanotransferase (CGTase) from Bacilus firmus was optimized in shake flasks using a statistical experimental design approach. Two level complete factorial designs in three variables were used for media optimization. The maximum CGTase activity obtained was 7.05 U ml(-1) with 21 g l(-1) corn starch, 22.6 g l(-1) yeast extract, 21.9 g l(-1) Pharmamedia, and an initial pH of 9.8. The optimized medium resulted in about a 20-fold increase in the enzyme activity as compared to that obtained in the basal medium. (C) 1998 Elsevier Science Inc.

引用

页码：288 / 291

页数：4

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