nNOS is involved in estrogen mediated neuroprotection in neuroblastoma cells

被引：21

作者：

Wen, Y

Perez, EJ

Green, PS

Sarkar, SN

Simpkins, JW

机构：

[1] Univ N Texas, Hlth Sci Ctr, Dept Pharmacol & Neurosci, Ft Worth, TX 76107 USA

[2] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63108 USA

来源：

NEUROREPORT | 2004年 / 15卷 / 09期

关键词：

cGMP; estrogen; neuroprotection; nitric oxide; nNOS;

D O I：

10.1097/01.wnr.0000131674.92694.96

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Estrogens exert neuroprotective activity in both in vivo and in vitro model systems. Herein, we report that both 17beta-estradiol and low concentrations of nitric oxide (NO) attenuate hydrogen peroxide (H2O2) induced toxicity in SK-N-SH cells, which express the neuronal nitric oxide synthase (nNOS). 17beta-estradiol rapidly induced an increase in NO levels. A nNOS inhibitor was able to block the neuroprotection of 17beta-estradiol. Cyclic guanylyl mono-phosphate (cGMP) also protected against H2O2 induced toxicity, while NO's protection was attenuated by ODQ a soluble guanylyl cyclase (sGC) inhibitor. In SK-N-SH cells, the major estrogen receptor isoforms is estrogen receptor beta. Our current study suggests that increased activity of nNOS may be involved in the neuroprotection conferred by 17beta-estradiol.

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收藏

页码：1515 / 1518

页数：4

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