Performance of columns packed with the new shell particles, Kinetex-C18

被引：206

作者：

Gritti, Fabrice ^{[1
]}

Leonardis, Irene ^{[1
]}

Shock, David ^{[2
]}

Stevenson, Paul ^{[2
]}

Shalliker, Andrew ^{[2
]}

Guiochon, Georges ^{[1
]}

机构：

[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA

[2] Univ Western Sydney, Australian Ctr Res Separat Sci, Penrith, NSW 1797, Australia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 10期

基金：

美国国家科学基金会;

关键词：

Column packing technology; Shell particles; Van Deemter plot; HETP; Kinetex-C-18; Halo-C-18; Anthracene; Naphtho[2,3-a]pyrene; Bradykinin; Insulin; Lyzozyme; Acetonitrile; MASS-TRANSFER KINETICS; POROUS SILICA MICROSPHERES; LIQUID-CHROMATOGRAPHY; DIFFUSION-COEFFICIENTS; UNSORBED SOLUTES; PLATE HEIGHT; GAS; EFFICIENCY; EQUATION; DIAMETER;

D O I：

10.1016/j.chroma.2009.12.079

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The performance of the new Kinetex-C-18 column was investigated. Packed with a new brand of porous shell particles, this column has an outstanding efficiency. Once corrected for the contribution of the instrument extra column volume, the minimum values of the reduced plate heights for a number of low molecular weight compounds (e.g., anthracene and naphtho[2,3-a]pyrene) were between 1.0 and 1.3, breaking the legendary record set 3 years ago by Halo-C-18 packed columns. The liquid-solid mass transfer of proteins (e.g., insulin and lyzozyme) is exceptionally fast on Kinetex-C-18 much faster than on the Halo-C-18 column. The different contributions of dispersion and mass transfer resistances to the column efficiency were determined and discussed. The possible reasons for this extremely high column efficiency are discussed. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1589 / 1603

页数：15

共 47 条

[1]

[Anonymous], 2008, 32 INT S HIGH PERF L

[2]

BEE C, 2003, AICHE J, V49, P642

[3] Structure of human insulin monomer in water/acetonitrile solution [J].