pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin

被引：7

作者：

Vanlandschoot, P ^{[1
]}

Beirnaert, E ^{[1
]}

Grooten, J ^{[1
]}

Jou, WM ^{[1
]}

Fiers, W ^{[1
]}

机构：

[1] State Univ Ghent VIB, Mol Biol Lab, B-9000 Ghent, Belgium

来源：

ARCHIVES OF VIROLOGY | 1998年 / 143卷 / 02期

关键词：

D O I：

10.1007/s007050050282

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HA0s molecules due to a low intracellular pH encountered during secretion.

引用

页码：227 / 239

页数：13

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