Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth

被引:533
作者
Mattson, MP [1 ]
Haddon, RC
Rao, AM
机构
[1] Univ Kentucky, Sanders Brown Res Ctr Aging, Lexington, KY 40536 USA
[2] Univ Kentucky, Dept Anat & Neurobiol, Lexington, KY 40536 USA
[3] NIA, Neurosci Lab, Baltimore, MD 21224 USA
[4] Univ Kentucky, Dept Chem & Phys, Lexington, KY 40506 USA
[5] Univ Kentucky, Ctr Appl Energy Res, Lexington, KY 40511 USA
[6] Univ Kentucky, Dept Phys & Astron, Lexington, KY 40511 USA
基金
美国国家科学基金会;
关键词
brain; growth cones; hippocampus; hydroxynonenal; nanotechnology;
D O I
10.1385/JMN:14:3:175
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Carbon nanotubes are strong, flexible, conduct electrical current, and can be functionalized with different molecules, properties that may be useful in basic and applied neuroscience research. We report the first application of carbon nanotube technology to neuroscience research. Methods were developed for growing embryonic rat-brain neurons on multiwalled carbon nanotubes. On unmodified nanotubes, neurons extend only one or two neurites, which exhibit very few branches. In contrast, neurons grown on nanotubes coated with the bioactive molecule 4-hydroxynonenal elaborate multiple neurites, which exhibit extensive branching. These findings establish the feasability of using nanotubes as substrates for nerve cell growth and as probes of neuronal function at the nanometer scale.
引用
收藏
页码:175 / 182
页数:8
相关论文
共 29 条
  • [1] Continuous production of aligned carbon nanotubes: a step closer to commercial realization
    Andrews, R
    Jacques, D
    Rao, AM
    Derbyshire, F
    Qian, D
    Fan, X
    Dickey, EC
    Chen, J
    [J]. CHEMICAL PHYSICS LETTERS, 1999, 303 (5-6) : 467 - 474
  • [2] 4-hydroxynonenal triggers Ca2+ influx in isolated rat hepatocytes
    Carini, R
    Bellomo, G
    Paradisi, L
    Dianzani, MU
    Albano, E
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (03) : 772 - 776
  • [3] Solution properties of single-walled carbon nanotubes
    Chen, J
    Hamon, MA
    Hu, H
    Chen, YS
    Rao, AM
    Eklund, PC
    Haddon, RC
    [J]. SCIENCE, 1998, 282 (5386) : 95 - 98
  • [4] CHEMISTRY AND BIOCHEMISTRY OF 4-HYDROXYNONENAL, MALONALDEHYDE AND RELATED ALDEHYDES
    ESTERBAUER, H
    SCHAUR, RJ
    ZOLLNER, H
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 1991, 11 (01) : 81 - 128
  • [5] Self-oriented regular arrays of carbon nanotubes and their field emission properties
    Fan, SS
    Chapline, MG
    Franklin, NR
    Tombler, TW
    Cassell, AM
    Dai, HJ
    [J]. SCIENCE, 1999, 283 (5401) : 512 - 514
  • [6] Mechanisms and molecules that control growth cone guidance
    Goodman, CS
    [J]. ANNUAL REVIEW OF NEUROSCIENCE, 1996, 19 : 341 - 377
  • [7] Hamon MA, 1999, ADV MATER, V11, P834, DOI 10.1002/(SICI)1521-4095(199907)11:10<834::AID-ADMA834>3.0.CO
  • [8] 2-R
  • [9] Large-scale production of single-walled carbon nanotubes by the electric-arc technique
    Journet, C
    Maser, WK
    Bernier, P
    Loiseau, A
    delaChapelle, ML
    Lefrant, S
    Deniard, P
    Lee, R
    Fischer, JE
    [J]. NATURE, 1997, 388 (6644) : 756 - 758
  • [10] CALCIUM REGULATION OF THE NEURONAL GROWTH CONE
    KATER, SB
    MATTSON, MP
    COHAN, C
    CONNOR, J
    [J]. TRENDS IN NEUROSCIENCES, 1988, 11 (07) : 315 - 321