Presence and localization of CCK receptor subtypes in calf pancreas

被引：11

作者：

Morisset, J

Lainé, J

Bourassa, J

Lessard, M

Rome, V

Guilloteau, P

机构：

[1] Univ Sherbrooke, Fac Med, Serv Gastroenterol, Dept Pathol & Med, Sherbrooke, PQ J1H 5N4, Canada

[2] Inst Natl Rech Agronom, Lab Jeune Ruminant, Rennes, France

来源：

REGULATORY PEPTIDES | 2003年 / 111卷 / 1-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

calf; pancreas; CCK receptor subtypes; somatostatin; glucagon; insulin; ductal cells;

D O I：

10.1016/S0167-0115(02)00261-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study was undertaken to confirm the presence of CCK receptor subtypes in calf pancreas and establish their cellular localization. Using specific antibodies against CCKA and CCKB receptors, somatostatin, glucagon and insulin, we were able to confirm by Western blot the presence of both CCK receptor protein subtypes in the calf pancreas as a 80-85-kDa CCKA receptor and 40-45-kDa CCKB receptor. By immunofluorescence, the CCKB receptor colocalizes with the islets' somatostatin delta cells, confirming what was previously shown in other species, as well as on ductal cells. We could not reproduce in the calf its colocalization with glucagon alpha cells as observed in human and rat. Any specific localization of CCKA receptors with our multiple antibodies failed. Our observation that the CCKB receptor subtype is specifically localized on pancreatic delta cells as well as on ductal cells lets us support the hypothesis that in this species, CCK could be involved in somatostatin metabolism as well as hydrelatic secretion; its effect on enzyme secretion would be indirect. (C) 2002 Elsevier Science B.V All rights reserved.

引用

页码：103 / 109

页数：7

共 24 条

[1] Ontogeny and species differences in the pancreatic expression and localization of the CCKA receptors [J].