In vitro biomarker discovery for atherosclerosis by proteomics

被引:79
作者
Fach, EM [1 ]
Garulacan, LA [1 ]
Gao, J [1 ]
Xiao, Q [1 ]
Storm, SM [1 ]
Dubaquie, YP [1 ]
Hefta, SA [1 ]
Opiteck, GJ [1 ]
机构
[1] Bristol Myers Squibb Co, Pharmaceut Res Inst, Dept Clin Discovery, Princeton, NJ 08543 USA
关键词
D O I
10.1074/mcp.M400160-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this study was to identify in vitro and then prioritize a tractable set of protein biomarker candidates of atherosclerosis that may eventually be developed to measure the extent, progression, regression, and stability of atherosclerotic lesions. A study was conducted using an in vitro "foam cell" model based on the stimulation of differentiated THP1 cells with oxidized low-density lipoprotein ( oxidized LDL) as compared with low-density lipoprotein ( LDL). Analysis of the proteins contained in the cell supernatant using proteome scanning technology identified 59 proteins as being increased, 57 with no statistically measurable difference, and 17 decreasing in abundance following treatment with oxidized LDL, as compared with LDL. From the up-regulated list, proteins were prioritized based on their analytical confidence as well as their relevance to atherosclerosis pathways. Within the group of increased abundance, seven families of proteins were of particular interest: fatty acid-binding proteins, chitinase-like enzymes, cyclophilins, cathepsins, proteoglycans, urokinase-type plasminogen activator receptor, and a macrophage scavenger receptor.
引用
收藏
页码:1200 / 1210
页数:11
相关论文
共 70 条
  • [1] ACTON SL, 1994, J BIOL CHEM, V269, P21003
  • [2] SELECTIVE ASSAY FOR CYPA AND CYPB IN HUMAN BLOOD USING HIGHLY SPECIFIC ANTIPEPTIDE ANTIBODIES
    ALLAIN, F
    BOUTILLON, C
    MARILLER, C
    SPIK, G
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 178 (01) : 113 - 120
  • [3] Serum chitotriosidase activity is increased in subjects with atherosclerosis disease
    Artieda, M
    Cenarro, A
    Gañán, A
    Jericó, I
    Gonzalvo, C
    Casado, JM
    Vitoria, I
    Puzo, J
    Pocoví, M
    Civeira, F
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2003, 23 (09) : 1645 - 1652
  • [4] Blockade of endothelin receptors markedly reduces atherosclerosis in LDL receptor deficient mice: role of endothelin in macrophage foam cell formation
    Babaei, S
    Picard, P
    Ravandi, A
    Monge, JC
    Lee, TC
    Cernacek, P
    Stewart, DJ
    [J]. CARDIOVASCULAR RESEARCH, 2000, 48 (01) : 158 - 167
  • [5] High sensitivity C-reactive protein: An emerging role in cardiovascular risk assessment
    Benzaquen, LR
    Yu, H
    Rifai, N
    [J]. CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES, 2002, 39 (4-5) : 459 - 497
  • [6] Plasma concentrations and genetic variation of matrix metalloproteinase 9 and prognosis of patients with cardiovascular disease
    Blankenberg, S
    Rupprecht, HJ
    Poirier, O
    Bickel, C
    Smieja, M
    Hafner, G
    Meyer, J
    Cambien, F
    Tiret, L
    [J]. CIRCULATION, 2003, 107 (12) : 1579 - 1585
  • [7] Adipocyte fatty acid-binding protein, aP2, alters late atherosclerotic lesion formation in severe hypercholesterolemia
    Boord, JB
    Maeda, K
    Makowski, L
    Babaev, VR
    Fazio, S
    Linton, MF
    Hotamisligil, GS
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (10) : 1686 - 1691
  • [8] Strong induction of members of the chitinase family of proteins in atherosclerosis - Chitotriosidase and human cartilage gp-39 expressed in lesion macrophages
    Boot, RG
    van Achterberg, TAE
    van Aken, BE
    Renkema, GH
    Jacobs, MJHM
    Aerts, JMFG
    de Vries, CJM
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1999, 19 (03) : 687 - 694
  • [9] LIPOPROTEIN METABOLISM IN THE MACROPHAGE - IMPLICATIONS FOR CHOLESTEROL DEPOSITION IN ATHEROSCLEROSIS
    BROWN, MS
    GOLDSTEIN, JL
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 : 223 - 261
  • [10] Human monocyte-derived macrophages secrete two forms of proteoglycan-macrophage colony-stimulating factor that differ in their ability to bind low density lipoproteins
    Chang, MY
    Olin, KL
    Tsoi, C
    Wight, TN
    Chait, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (26) : 15985 - 15992