Analysis of peptides using N-methylpolyvinylpyridium as silica surface modifier for CE-ESI-MS

被引：17

作者：

Elhamili, Anisa ^{[1
]}

Wetterhall, Magnus ^{[1
]}

Sjodin, Marcus ^{[1
]}

Sebastiano, Roberto ^{[2
]}

Bergquist, Jonas ^{[1
]}

机构：

[1] Uppsala Univ, Dept Phys & Analyt Chem, SE-75124 Uppsala, Sweden

[2] Polytech Milano, Dept Chem Mat & Chem Engn Giulio Natta, Milan, Italy

来源：

ELECTROPHORESIS | 2010年 / 31卷 / 07期

基金：

瑞典研究理事会;

关键词：

CE; ESI; MS; N-Methylpolyvinylpyridinuim; Peptides; CAPILLARY-ZONE-ELECTROPHORESIS; FLIGHT MASS-SPECTROMETRY; TRIFUNCTIONAL DIAMINE; PROTEIN SEPARATIONS; COATINGS; POLYMER; WALL; DEACTIVATION; SALTS;

D O I：

10.1002/elps.200900536

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In this study, the N-methylpolyvinylpyridinuim polymer has for the first time been used as a silica surface modifier for CE in combination with ESI MS (CE-ESI-MS) The compatibility for ESI-MS was demonstrated by the analysis of peptides and protein digests. The N-methylpolyvinylpyridium surface interacts electrostatically with the ionized silanol groups, giving a cationic surface with a reversed EOF The surface modifier gave rapid and repeatable separations of peptides, proteins and protein digests at acidic pH for more than 4 h of continuous use The CE separation yielded peak efficiencies of up to 4.3 x 10(5) plates/m The surface coating is highly compatible with ESI and facilitates the separation and analysis of complex peptide mixtures as shown by the analysis of BSA digests.

引用

页码：1151 / 1156

页数：6

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