Nanoparticles that display short collagen-related peptides. Potent stimulation of human platelet aggregation by triple helical motifs

被引：16

作者：

Cejas, Mabel A. ^{[1
]}

Chen, Cailin ^{[1
]}

Kinney, William A. ^{[1
]}

Maryanoff, Bruce E. ^{[1
]}

机构：

[1] Johnson & Johnson Pharmaceut Res & Dev, Vasc Res Team, Spring House, PA 19477 USA

来源：

BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 04期

关键词：

D O I：

10.1021/bc070105s

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Collagen plays a key role in the activation and adhesion of blood platelets via their cell-surface receptors. Normally, collagen-related peptides (CRPs), even one as long as a 30-mer (10 Gly-Pro-Hyp (GPO) repeats), are unable to effectively express collagen's platelet-activating behavior. We attached two short CRPs, AcHN-(Gly-Pro-Hyp)(n)Gly-OH with n = 5 (1) and n = 10 (2), via the C-terminus to amino-functionalized latex nanoparticles to create a multimeric display of triple helical motifs. These nanomaterials were characterized by dynamic light scattering and environmental scanning electron microscopy. The nanoparticles bearing the 31-mer CRP sequence, 2, but not the 16-mer sequence, 1, effectively induced the aggregation of human platelets, with a potency level approaching that of native type I collagen. Our results highlight the importance of presenting triple helical CRP motifs of sufficient length on a suitable scaffold in order to stimulate platelets.

引用

页码：1025 / 1027

页数：3

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