Oxidation of high density lipoproteins - II. Evidence for direct reduction of lipid hydroperoxides by methionine residues of apolipoproteins AI and AII

被引:269
作者
Garner, B
Waldeck, AR
Witting, PK
Rye, KA
Stocker, P
机构
[1] Heart Res Inst, Biochem Grp, Sydney, NSW 2050, Australia
[2] Royal Adelaide Hosp, Cardiovasc Invest Unit, Adelaide, SA 5000, Australia
关键词
D O I
10.1074/jbc.273.11.6088
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human high density lipoproteins (HDL) can reduce cholesteryl ester hydroperoxides to the corresponding hydroxides (Sattler W,, Christison J, K,, and Stocker, R. (1995) Free Radical Biol, & Med. 18, 421-429), Here we demonstrate that this reducing activity extended to hydroperoxides of phosphatidylcholine, was similar in HDL2 and HDL3, was independent of arylesterase and lecithin:cholesteryl acyltransferase activity, was unaffected by sulfhydryl reagents, and was expressed by reconstituted particles containing apoAI, or apoAII only, as well as isolated human apoAI, Concomitant with the reduction of lipid hydroperoxides specific oxidized forms of apoAI and apoAII formed in blood-derived and reconstituted HDL, Similarly, specific oxidized forms of apoAI accumulated upon treatment of isolated apoAI with authentic cholesteryl linoleate hydroperoxide, These specific oxidized forms of apoAI and apoAII have been shown previously to contain Met sulfoxide (Met(O)) at Met residues and are also formed when HDL is exposed to Cu2+ Or soybean lipoxygenase, Lipid hydroperoxide reduction and the associated formation of specific oxidized forms of apoAI and apoAII were inhibited by solubilizing HDL with SDS or by pretreatment of HDL with chloramine T, The inhibitory effect of chloramine T was dose-dependent and accompanied by the conversion of specific Met residues of apoAI and apoAII into Met(O), Canine HDL, which contains apoAI as the predominant apolipoprotein and which lacks the oxidation-sensitive Met residues Met(112) and Met(148), showed much weaker lipid hydroperoxide reducing activity and lower extents of formation of oxidized forms of apoAI than human HDL, We conclude that the oxidation of specific Met residues of apoAI and apoAII to Met(O) plays a significant role in the 2-electron reduction of hydroperoxides of cholesteryl esters and phosphatidylcholine associated with human HDL.
引用
收藏
页码:6088 / 6095
页数:8
相关论文
共 43 条
[1]  
ALBERS JJ, 1981, J LIPID RES, V22, P1206
[2]  
ANANTHARAMAIAH GM, 1988, J LIPID RES, V29, P309
[3]   The role of oxidized lipoproteins in atherogenesis [J].
Berliner, JA ;
Heinecke, JW .
FREE RADICAL BIOLOGY AND MEDICINE, 1996, 20 (05) :707-727
[4]   BIOCHEMISTRY AND PHYSIOLOGICAL-ROLE OF METHIONINE SULFOXIDE RESIDUES IN PROTEINS [J].
BROT, N ;
WEISSBACH, H .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 223 (01) :271-281
[5]   Rapid reduction and removal of HDL- but not LDL-associated cholesteryl ester hydroperoxides by rat liver perfused in situ [J].
Christison, J ;
Karjalainen, A ;
Brauman, J ;
Bygrave, F ;
Stocker, R .
BIOCHEMICAL JOURNAL, 1996, 314 :739-742
[6]  
CHRISTISON JK, 1995, J LIPID RES, V36, P2017
[7]  
CHUNG H, 1982, J BIOL CHEM, V257, P2961
[8]  
CHUNGLIANG K, 1995, DRUG METAB DISPOS, V23, P935
[9]  
EDELSTEIN C, 1973, J BIOL CHEM, V248, P7653
[10]   OXIDATION OF METHIONINE RESIDUES IN PROTEINS OF ACTIVATED HUMAN-NEUTROPHILS [J].
FLISS, H ;
WEISSBACH, H ;
BROT, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (23) :7160-7164