Freeze-fracture electron microscopy of lipid membranes on colloidal polyelectrolyte multilayer coated supports

被引：30

作者：

Moya, S

Richter, W

Leporatti, S

Bäumler, HB

Donath, E

机构：

[1] Univ Leipzig, Inst Med Phys & Biophys, D-04103 Leipzig, Germany

[2] Univ Jena, Inst Ultrastruct Res, D-07740 Jena, Germany

[3] Humboldt Univ, Charite, Fac Med, Inst Transfus Med, D-10098 Berlin, Germany

来源：

BIOMACROMOLECULES | 2003年 / 4卷 / 03期

关键词：

D O I：

10.1021/bm034013r

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipid membranes were assembled on polyelectrolyte (PE)-coated colloidal particles. The assembly was studied by means of confocal microscopy, flow cytometry, scanning force microscopy, and freeze-fracture electron microscopy. A homogeneous lipid coverage was established within the limits of optical resolution. Flow cytometry showed that the lipid coverage was uniform. Freeze-fracture electron microscopy revealed that the lipid was adsorbed as a bilayer, which closely followed the surface profile of the polyelectrolyte support. Additional adsorption of polyelectrolyte layers on top of the lipid bilayer introduced inhomogeneities as evident from jumps in the fracture plane. Characteristic lipid multilayers have not been seen with freeze-fracture electron microscopy.

引用

页码：808 / 814

页数：7

共 20 条

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