Purification and characterization of NADPH-dependent acetoacetyl-CoA reductase from Methylobacterium extorquens

被引：6

作者：

Belova, LL ^{[1
]}

Sokolov, AP ^{[1
]}

Sidorov, IA ^{[1
]}

Trotsenko, YA ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Physiol Microorganisms, Pushchino 142292, Moscow Region, Russia

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 156卷 / 02期

关键词：

acetoacetyl-CoA reductase; methylotroph; Methylobacterium extorquens; poly-3-hydroxybutyrate;

D O I：

10.1111/j.1574-6968.1997.tb12740.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

NADPH-dependent acetoacetyl-CoA (AcAc-CoA) reductase (EC 1.1.1.36) was purified to electrophoretic homogeneity from the methylotroph Methylobacterium extorquens - a producer of poly-3-hydroxybutyrate. The enzyme has an M-r of 141000 and consists of four identical subunits (M-r 31000) and demonstrates absolute specificity for NADPH as cofactor. NADPH-AcAc-CoA reductase is inhibited by NADPH, AcAc-CoA, NADP and NAD but is activated by isocitrate and ATP. The calculated K-m values were 11.6 and 41 mu M for AcAc-CoA and NADPH, respectively. The results suggest that this enzyme plays a more important role in the coordinated operation of the tricarboxylic acid cycle and poly-3-hydroxybutyrate synthesis in M. extorquens than in the taxonomically related M. rhodesianum MB 126.

引用

页码：275 / 279

页数：5

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