PER-1- and OXA-10-like β-lactamases in ceftazidime-resistant Pseudomonas aeruginosa isolates from intensive care unit patients in Istanbul, Turkey

被引:33
作者
Aktas, Z [1 ]
Poirel, L
Salcioglu, M
Özcan, PE
Midilli, K
Bal, Ç
Ang, Ö
Nordmann, P
机构
[1] Istanbul Univ, Istanbul Fac Med, Dept Microbiol & Clin Microbiol, Istanbul, Turkey
[2] Fac Med Paris Sud, Assistance Publ Hop Paris, Hop Bicetre, Serv Bacteriol Virol, Le Kremlin Bicetre, France
[3] Istanbul Univ, Istanbul Fac Med, Dept Anesthesiol, Istanbul, Turkey
[4] Istanbul Univ, Cerrahpasa Fac Med, Dept Microbiol & Clin Microbiol, Istanbul, Turkey
关键词
beta-lactamase; ceftazidime resistance; intensive care unit; OXA-10; PER-1; Pseudomonas aeruginosa;
D O I
10.1111/j.1469-0691.2004.01067.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The presence of PER-1- and OXA-10-like beta-lactamases was investigated by PCR in 49 ceftazidime-resistant Pseudomonas aeruginosa isolates from patients hospitalised in the 24-bed general intensive care unit of the Istanbul Faculty of Medicine during a 12-month period between February 1999 and February 2000. The clonal relatedness of the isolates was investigated by random amplified polymorphic DNA (RAPD) analysis, and the sequences of the PER-1 and OXA genes from all isolates were determined. The rates of resistance of the isolates to imipenem, aztreonam and cefepime were 98%, 92% and 96%, respectively, and to piperacillin and piperacillin-tazobactam were 41% and 37%, respectively. Using the double-disk synergy test, 37% (18/49) of the isolates were identified as extended-spectrum beta-lactamase producers. The PER-1 gene was identified in 86% (42/49) and the OXA-10 gene in 55% (27/49) of the ceftazidime-resistant isolates. Of isolates carrying the PER-1 gene, 48% (20/42) also carried the OXA-10 gene. The respective nucleotide sequences were identical for each isolate. Sixteen RAPD patterns were detected among the PER-1-positive isolates, but 60% (25/42) of the PER-1-positive isolates belonged to two distinct patterns, while the remainder exhibited a wide clonal diversity. The results indicated that the prevalence of PER-1- and OXA-10-like beta-lactamases remains high among ceftazidime-resistant P. aeruginosa isolates in Turkey.
引用
收藏
页码:193 / 198
页数:6
相关论文
共 41 条
  • [1] Role of quantitative cultures and microscopic examinations of endotracheal aspirates in the diagnosis of pulmonary infections in ventilated patients
    Albert, S
    Kirchner, J
    Thomas, H
    Behne, M
    Schur, J
    Brade, V
    [J]. JOURNAL OF HOSPITAL INFECTION, 1997, 37 (01) : 25 - 37
  • [2] BAHAR UG, 2003, CLIN MICROBIOL INFEC, V9, P306
  • [3] Cross-colonisation with Pseudomonas aeruginosa of patients in an intensive care unit
    Bergmans, DCJJ
    Bonten, MJM
    van Tiel, FH
    Gaillard, CA
    van der Geest, S
    Wilting, RM
    de Leeuw, PW
    Stobberingh, EE
    [J]. THORAX, 1998, 53 (12) : 1053 - 1058
  • [4] PER-1 β-lactamase-producing Pseudomonas aeruginosa in an intensive care unit
    Claeys, G
    Verschraegen, G
    de Baere, T
    Vaneechoutte, M
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 45 (06) : 924 - 925
  • [5] AMANTADINE PROPHYLAXIS FOR HEALTH-CARE WORKERS - UNANSWERED QUESTIONS
    COX, RA
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1991, 27 (01) : 1 - 3
  • [6] TRANSFERABLE PRODUCTION OF PER-1 BETA-LACTAMASE IN PSEUDOMONAS-AERUGINOSA
    DANEL, F
    HALL, IMC
    GUR, D
    AKALIN, HE
    LIVERMORE, DM
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1995, 35 (02) : 281 - 294
  • [7] OXA-15, an extended-spectrum variant of OXA-2 beta-lactamase, isolated from a Pseudomonas aeruginosa strain
    Danel, F
    Hall, LMC
    Gur, D
    Livermore, DM
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (04) : 785 - 790
  • [8] OXA-17, a further extended-spectrum variant of OXA-10 β-lactamase, isolated from Pseudomonas aeruginosa
    Danel, F
    Hall, LMC
    Duke, B
    Gur, D
    Livermore, DM
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (06) : 1362 - 1366
  • [9] Prospective survey of β-lactamases produced by ceftazidime-resistant Pseudomonas aeruginosa isolated in a French hospital in 2000
    De Champs, C
    Poirel, L
    Bonnet, R
    Sirot, D
    Chanal, C
    Sirot, J
    Nordmann, P
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) : 3031 - 3034
  • [10] Docquier JD, 2001, EMERG INFECT DIS, V7, P910