Use of rpsL as a Counterselectable Marker in Borrelia burgdorferi

被引：14

作者：

Drecktrah, Dan ^{[1
]}

Douglas, J. Miles ^{[1
]}

Samuels, D. Scott ^{[1
,2
]}

机构：

[1] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA

[2] Univ Montana, Ctr Biomol Struct & Dynam, Missoula, MT 59812 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 03期

基金：

美国国家卫生研究院;

关键词：

LYME-DISEASE SPIROCHETE; PEPTIDYL-TRANSFER-RNA; ESCHERICHIA-COLI; STREPTOMYCIN; RESISTANCE; PROTEIN; TRANSFORMATION; RIBOSOMES; GENETICS; AGENT;

D O I：

10.1128/AEM.02172-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.

引用

收藏

页码：985 / 987

页数：3

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