Modifications in the purification protocol of Celosia cristata antiviral proteins lead to protein that can be N-terminally sequenced

被引：20

作者：

Gholizadeh, A ^{[1
]}

Kapoor, HC ^{[1
]}

机构：

[1] Indian Agr Res Inst, Div Biochem, New Delhi 110012, India

来源：

PROTEIN AND PEPTIDE LETTERS | 2004年 / 11卷 / 06期

关键词：

antiviral; proteins; glycoproteins; Celosia; purification;

D O I：

10.2174/0929866043406210

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants antiviral proteins are being used as anticancer agents and inhibit other viral diseases in humans. We modified the purification protocol of the two N-terminally blocked antiviral glycoproteins, CCP-25 and CCP-27. purified from the leaves of Celosia cristata. This not only gave rise to single pure samples with few steps of purification but also resulted in N-terminally free proteins. The extra purity of the samples was analyzed by reverse phase HPLC. Deglycosylation studies of CCP-25 with PNGase F enzyme revealed that its asparagine or asparagine-linked glycon contents are negligible. Partial N-terminal sequence of the CCP-25 showed the sequence (ANDIS), which seems to be conserved among plant antiviral proteins.

引用

页码：555 / 561

页数：7

共 19 条

[1]

ANTHONY L, 1985, BIOCHEM, V24, P4665

[2]

ASHRAF G, 2004, J PLANT BIOCHEM BIOT, V13, P13

[3] An antiviral protein from Bougainvillea spectabilis roots;: Purification and characterisation [J].

Balasaraswathi, R ;

Sadasivam, S ;

Ward, M ;

Walker, JM .

PHYTOCHEMISTRY, 1998, 47 (08) :1561-1565

[4] Purification and properties of growth stage-dependent antiviral proteins from the leaves of Celosia cristata [J].

Balasubrahmanyam, A ;

Baranwal, VK ;

Lodha, ML ;

Varma, A ;

Kapoor, HC .

PLANT SCIENCE, 2000, 154 (01) :13-21

[5]

BARANWAL VK, 1992, PLANT PATHOL, V41, P633, DOI 10.1111/j.1365-3059.1992.tb02464.x

[6] Characteristics of a virus inhibitor from the leaf extract of Celosia cristata [J].

Baranwal, VK ;

Verma, HN .

PLANT PATHOLOGY, 1997, 46 (04) :523-529

[7] Resistance to geminivirus infection by virus-induced expression of dianthin in transgenic plants [J].

Hong, Y ;

Saunders, K ;

Hartley, MR ;

Stanley, J .

VIROLOGY, 1996, 220 (01) :119-127

[8] PURIFICATION AND PROPERTIES OF A SECOND ANTI-VIRAL PROTEIN FROM PHYTOLACCA-AMERICANA WHICH INACTIVATES EUKARYOTIC RIBOSOMES [J].

IRVIN, JD ;

KELLY, T ;

ROBERTUS, JD .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1980, 200 (02) :418-425

[9]

Jiang GY, 1999, ACTA BOT SIN, V41, P334

[10]

KUBO S, 1990, Annals of the Phytopathological Society of Japan, V56, P481, DOI 10.3186/jjphytopath.56.481

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