Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

被引：48

作者：

Ijima, Y ^{[1
]}

Matoishi, K ^{[1
]}

Terao, Y ^{[1
]}

Doi, N ^{[1
]}

Yanagawa, H ^{[1
]}

Ohta, H ^{[1
]}

机构：

[1] Keio Univ, Dept Biosci & Informat, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

来源：

CHEMICAL COMMUNICATIONS | 2005年 / 07期

关键词：

D O I：

10.1039/b416398b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

引用

页码：877 / 879

页数：3

共 17 条

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