Mechanistic Insight into Enzymatic Glycosyl Transfer with Retention of Configuration through Analysis of Glycomimetic Inhibitors

被引：81

作者：

Errey, James C. ^{[1
]}

Lee, Seung Seo ^{[1
]}

Gibson, Robert P. ^{[2
]}

Fleites, Carlos Martinez ^{[2
]}

Barry, Conor S. ^{[1
]}

Jung, Pierre M. J. ^{[3
]}

O'Sullivan, Anthony C. ^{[3
]}

Davis, Benjamin G. ^{[1
]}

Davies, Gideon J. ^{[2
]}

机构：

[1] Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England

[2] Univ York, Dept Chem, York YO10 5YW, N Yorkshire, England

[3] Syngenta AG, CH-4332 Stein, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 07期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

bisubstrate mimetic; enzymes; glycosyltransferases; internal return; trehaloses; ANTIBIOTIC VALIDAMYCINS; CRYSTAL-STRUCTURE; TREHALOSE; BIOSYNTHESIS; SOLVOLYSIS; COMPLEX; DONOR;

D O I：

10.1002/anie.200905096

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Structural "valid"-ation: The mechanism of enzyme-catalyzed glycosyl transfer with retention of anomeric configuration continues to baffle, a situation compounded by the lack of insightful 3-D structures of ternary enzyme complexes. Synthesis and multi-dimensional kinetic analysis of validoxylamine derivatives are used to access the 3-D structure of a ternary complex (see picture; U = uridyl) providing insight into the geometry and donor-acceptor interplay at the glycosyltransfer site. © 2010 Wiley-VCH Verlag GmbH &. Co. KGaA.

引用

页码：1234 / 1237

页数：4

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