Illuminating plant biology: using fluorescent proteins for high-throughput analysis of protein localization and function in plants

被引：16

作者：

DeBlasio, Stacy L. ^{[1
]}

Sylvester, Anne W.

Jackson, David ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

来源：

BRIEFINGS IN FUNCTIONAL GENOMICS | 2010年 / 9卷 / 02期

基金：

美国国家科学基金会;

关键词：

fluorescent proteins; maize; Arabidopsis; high-throughput techniques; global expression; functional profiling; TARGETED GENE-EXPRESSION; ENHANCER TRAP LINES; CELL-TO-CELL; ARABIDOPSIS-THALIANA; FUSION PROTEIN; DEVELOPMENTAL PLASTICITY; TRANSGENE EXPRESSION; EPIDERMAL-CELLS; APICAL MERISTEM; ROOT-MERISTEM;

D O I：

10.1093/bfgp/elp060

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

First discovered in jellyfish, fluorescent proteins (FPs) have been successfully optimized for use as effective biomarkers within living plant cells. When exposed to light, FPs fused to a protein or regulatory element will fluoresce, and non-invasively mark expression and protein localization, which allows for the in vivo monitoring of diverse cellular processes. In this review, we discuss how FP technology has evolved from small-scale analysis of individual genes to more high-throughput techniques for global expression and functional profiling in plants.

引用

页码：129 / 138

页数：10

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