Expression of human estrogen sulfotransferase in Salmonella typhimurium:: differences between hHST and hEST in the enantioselective activation of 1-hydroxyethylpyrene to a mutagen

被引：23

作者：

Hagen, M

Pabel, U

Landsiedel, R

Bartsch, I

Falany, CN

Glatt, H ^{[1
]}

机构：

[1] German Inst Human Nutr, D-14558 Potsdam, Germany

[2] Univ Mainz, Inst Toxicol, D-55131 Mainz, Germany

[3] Univ Alabama, Dept Pharmacol & Toxicol, Birmingham, AL USA

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1998年 / 109卷 / 1-3期

关键词：

Ames test; benzylic alcohols; enantioselective metabolism; heterologous expression; sulfotransferases;

D O I：

10.1016/S0009-2797(97)00136-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various human sulfotransferases (hP-PST, hM-PST, hHST) and rat sulfotransferases (rPST-IV, rHSTa) have already been expressed in Ames' Salmonella strains (in particular in TA1538), Now a further strain, TA1538-hEST, which expresses the human estrogen sulfotransferase (hEST), has been constructed. This strain activated the primary benzylic alcohol 1-hydroxymethylpyrene (1-HMP) and the secondary benzylic alcohol 1-hydroxyethylpyrene (1-HEP) to mutagens. Human sulfotransferases hEST and hHST both activated 1-HEP, but they differed substantially in their enantioselectivity for this compound. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：249 / 253

页数：5

共 7 条

[1] PURIFICATION AND IMMUNOCHEMICAL CHARACTERIZATION OF A MALE-SPECIFIC RAT-LIVER ESTROGEN SULFOTRANSFERASE [J].