Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria

被引：74

作者：

Piwowarski, J

Grzechnik, P

Dziembowski, A

Dmochowska, A

Minczuk, M

Stepien, PP

机构：

[1] Warsaw Univ, Dept Genet, PL-02106 Warsaw, Poland

[2] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 329卷 / 05期

关键词：

mitochondria; polynucleotide phosphorylase; PNPase; RNA degradation;

D O I：

10.1016/S0022-2836(03)00528-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human gene encoding a polynucleotide phosphorylase (hPNPase) has been recently identified as strongly up-regulated in two processes leading to irreversible arrest of cell division: progeroid senescence and terminal differentiation. Here, we demonstrate that the hPNPase is localized in mitochondria. Our finding suggests the involvement of mitochondrial RNA metabolism in cellular senescence. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：853 / 857

页数：5

相关论文

共 27 条

[1] Extensive feature detection of N-terminal protein sorting signals [J].

Bannai, H ;

Tamada, Y ;

Maruyama, O ;

Nakai, K ;

Miyano, S .

BIOINFORMATICS, 2002, 18 (02) :298-305

[2] The Escherichia coli RNA degradosome:: structure, function and relationship to other ribonucleolytic multienyzme complexes [J].

Carpousis, AJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :150-155

[3]

Chawla-Sarkar M, 2001, CLIN CANCER RES, V7, P1821

[4]

CLAROS MG, 1996, EUR J BIOCHEM, V241, P770, DOI DOI 10.1111/J.1432-1033.1996.00779.X

[5] THE NOVEL NUCLEAR GENE DSS-1 OF SACCHAROMYCES-CEREVISIAE IS NECESSARY FOR MITOCHONDRIAL BIOGENESIS [J].

DMOCHOWSKA, A ;

GOLIK, P ;

STEPIEN, PP .

CURRENT GENETICS, 1995, 28 (02) :108-112

[6] The yeast mitochondrial degradosome - Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism [J].

Dziembowski, A ;

Piwowarski, J ;

Hoser, R ;

Minczuk, M ;

Dmochowska, A ;

Siep, M ;

van der Spek, H ;

Grivell, L ;

Stepien, PP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (03) :1603-1611

[7] The yeast nuclear gene DSS1 which codes for a putative RNase II, is necessary for the function of the mitochondrial degradosome in processing and turnover of RNA [J].

Dziembowski, A ;

Malewicz, M ;

Minczuk, M ;

Golik, P ;

Dmochowska, A ;

Stepien, PP .

MOLECULAR AND GENERAL GENETICS, 1998, 260 (01) :108-114

[8] Genetic and biochemical approaches for analysis of mitochondrial degradosome from Saccharomyces cerevisiae [J].

Dziembowski, A ;

Stepien, PP .

RIBONUCLEASES, PT B, 2001, 342 :367-378

[9] Predicting subcellular localization of proteins based on their N-terminal amino acid sequence [J].

Emanuelsson, O ;

Nielsen, H ;

Brunak, S ;

von Heijne, G .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (04) :1005-1016

[10] THE GENE RESPONSIBLE FOR WERNER SYNDROME MAY BE A CELL-DIVISION COUNTING GENE [J].

FARAGHER, RGA ;

KILL, IR ;

HUNTER, JAA ;

POPE, FM ;

TANNOCK, C ;

SHALL, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :12030-12034