Identification of Novel Spinal Cholinergic Genetic Subtypes Disclose Chodl and Pitx2 as Markers for Fast Motor Neurons and Partition Cells

被引：86

作者：

Enjin, Anders ^{[1
]}

Rabe, Nadine ^{[1
]}

Nakanishi, Stan T. ^{[2
]}

Vallstedt, Anna ^{[1
]}

Gezelius, Henrik ^{[1
]}

Memic, Fatima ^{[1
]}

Lind, Magnus ^{[1
]}

Hjalt, Tord ^{[3
]}

Tourtellotte, Warren G. ^{[4
]}

Bruder, Carl ^{[5
]}

Eichele, Gregor ^{[6
]}

Whelan, Patrick J. ^{[2
]}

Kullander, Klas ^{[1
]}

机构：

[1] Uppsala Univ, Dept Neurosci, S-75123 Uppsala, Sweden

[2] Univ Calgary, Hotchkiss Brain Inst, Calgary, AB T2N 4N1, Canada

[3] Lund Univ, Dept Expt Med Res, BMC B12, S-22184 Lund, Sweden

[4] Northwestern Univ, Dept Pathol, Feinberg Sch Med, Chicago, IL 60611 USA

[5] Univ Alabama Birmingham, Dept Genet, Birmingham, AL 35294 USA

[6] Max Planck Inst Expt Endocrinol, D-30625 Hannover, Germany

来源：

JOURNAL OF COMPARATIVE NEUROLOGY | 2010年 / 518卷 / 12期

基金：

英国医学研究理事会;

关键词：

mouse genetics; neuronal network; interneuron; motor neuron; spinal cord; genetic screen; TRANSCRIPTION FACTOR EGR3; SLOW MUSCLE REGIONS; MEDIAL GASTROCNEMIUS; UNIT TYPES; GENERATION PATTERNS; NEUROTROPHIC FACTOR; C-TERMINALS; FAST-TWITCH; BRAIN-STEM; ADULT-RAT;

D O I：

10.1002/cne.22332

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Spinal cholinergic neurons are critical for motor function in both the autonomic and somatic nervous systems and are affected in spinal cord injury and in diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy. Using two screening approaches and in situ hybridization, we identified 159 genes expressed in typical cholinergic patterns in the spinal cord. These include two general cholinergic neuron markers, one gene exclusively expressed in motor neurons, and nine genes expressed in unknown sub-types of somatic motor neurons. Further, we present evidence that chondrolectin (Chodl) is expressed by fast motor neurons and that estrogen-related receptor beta (ERR beta) is a candidate marker for slow motor neurons. In addition, we suggest paired-like homeodomain transcription factor 2 (Pitx2) as a marker for cholinergic partition cells. J. Comp. Neurol. 518:2284-2304, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：2284 / 2304

页数：21

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