A 70 amino acid region within the semaphorin domain activates specific cellular response of semaphorin family members

被引：131

作者：

Koppel, AM ^{[1
]}

Feiner, L ^{[1
]}

Kobayashi, H ^{[1
]}

Raper, JA ^{[1
]}

机构：

[1] UNIV PENN,SCH MED,DEPT NEUROSCI,PHILADELPHIA,PA 19104

来源：

NEURON | 1997年 / 19卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0896-6273(00)80369-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The semaphorin family contains secreted and transmembrane signaling proteins that function in the nervous, immune, and cardiovascular systems. Chick collapsin-1 is a repellent for specific growth cones. Two other secreted members of the semaphorin family, collapsin-2 and -3, are structurally similar to collapsin-1 but have different biological activities. Semaphorins contain a 500 amino acid family signature semaphorin domain. We show in this study that (1) the semaphorin domain of collapsin-1 is both necessary and sufficient for biological activity, (2) the semaphorin domain contains a 70 amino acid region that specifies the biological activity of the three family members, and (3) the positively charged carboxy terminus potentiates activity without affecting specificity. We propose that semaphorins interact with their receptors through two independent binding sites: one that mediates the biological response and one that potentiates it.

引用

页码：531 / 537

页数：7

共 19 条

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