Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis

被引：126

作者：

Zhang, CH ^{[1
]}

Mei, XG ^{[1
]}

Liu, L ^{[1
]}

Yu, LJ ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 19期

关键词：

combined-elicitation; elicitor; paclitaxel (taxol); Taxus chinensis;

D O I：

10.1023/A:1005684901329

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l(-1), 60 mu M methyl jasmonate and 30 mu M Ag+ resulted in the greatest paclitaxel production, at 25 mg l(-1) in the cultures, being almost 40 times higher than that of the control culture, 10 times higher than that of the culture exposed to Ag+, 6 times higher than that of the culture elicited by chitosan and almost double that of the culture elicited by methyl jasmonate.

引用

页码：1561 / 1564

页数：4

共 14 条

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