Cloning and functional expression in Escherichia coli of a cDNA encoding cardenolide 16′-O-glucohydrolase from Digitalis lanata Ehrh.

被引：7

作者：

Framm, JJ

Peterson, A

Thoeringer, C

Pangert, A

Hornung, E

Feussner, I

Luckner, M

Lindemann, P

机构：

[1] Univ Halle Wittenberg, Inst Pharmazeut Biol, D-06120 Halle, Germany

[2] Biozentrum, D-06120 Halle, Germany

[3] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

来源：

PLANT AND CELL PHYSIOLOGY | 2000年 / 41卷 / 11期

关键词：

Digitalis lanata; glucosyl hydrolase (EC 3.2.1.21); lanatoside; Scrophulariaceae;

D O I：

10.1093/pcp/pcd060

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A clone of cardenolide 16'-O-glucohydrolase cDNA (CGH I) was obtained from Digitalis lanata which encodes a protein of 642 amino acids (calculated molecular mass 73.2 kDa), The amino acid sequence derived from CGH I showed high homology to a widely distributed family of beta -glucohydrolases (glycosyl hydrolases family 1). The recombinant CGH I protein produced in Escherichia coli had CGH I activity, CGH I mRNA was detected in leaves, flowers, stems and fruits of D. lanata.

引用

页码：1293 / 1298

页数：6

共 47 条

[1]

BARISCH H, 1972, PHARMAZIE, V27, P415

[2]

BARISCH H, 1972, PHARMAZIE, V27, P416

[3]

*BOEHR MANNH, 1990, DIG PROD BEI BM BOEH

[4]

*BOEHR MANNH, 1995, DIG SYST US GUID FIL