Mutation scanning methods for the analysis of parasite genes

被引：85

作者：

Gasser, RB ^{[1
]}

机构：

[1] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1997年 / 27卷 / 12期

关键词：

parasite genes; sequence variation; mutation scanning (detection) methods;

D O I：

10.1016/S0020-7519(97)00131-8

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Molecular variation is widespread in parasite populations, and its analysis has important implications for studying aspects relating to the function and organisation of genes, and the taxonomy, phylogeny and population genetics of parasites. This article reviews some PCR-based mutation scanning techniques that have advantages over currently used DNA methods for the analysis of genetic variation in parasites. The review is technical and describes briefly the principles of relevant techniques, examines some of their advantages and disadvantages and gives several examples for possible applications. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.

引用

页码：1449 / 1463

页数：15

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