Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomes

被引：89

作者：

Fukui, K ^{[1
]}

Nakayama, S

Ohmido, N

Yoshiaki, H

Yamabe, M

机构：

[1] Hokkaido Natl Agr Expt Stn, Joetsu 94301, Japan

[2] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 305, Japan

[3] Ishikawa Prefectural Agr Ctr, Kanazawa, Ishikawa 92001, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 1998年 / 96卷 / 3-4期

关键词：

Brassica rapa; Brassica nigra; Brassica oleracea; quantitative chromosome map; idiogram; FISH; 45s rDNA; systematic mapping;

D O I：

10.1007/s001220050744

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Chromosomes of the three diploid Brassica species, B. rapa (AA), B. nigra (BE) and B. oleracea (CC), were identified based on their morphological characteristics, especially on the condensation pattern appearing at the somatic pro-metaphase stage. The morphological features of the pro-metaphase chromosomes of the three Brassica spp. were quantified by imaging methods using chromosome image analyzing system II (CHIAS 2). As a result, quantitative chromosome maps or idiograms of the three diploid Brassica spp. were developed. The fluorescence in situ hybridization (FISH) method revealed the location of 45s rDNA (the 26s-5.8s-18s ribosomal RNA gene cluster) on the chromosomes involved. The number of 45s rDNA loci in the B. rapa, B. nigra and B. oleracea are five, three and two, respectively. The loci detected were systematically mapped on the idiograms of the three Brassica spp.

引用

页码：325 / 330

页数：6

共 29 条

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