Covalent immobilization of glucose oxidase on poly[1-(2-carboxyethyl)pyrrole] film for glucose sensing

被引：33

作者：

Kojima, K ^{[1
]}

Yamauchi, T ^{[1
]}

Shimomura, M ^{[1
]}

Miyauchi, S ^{[1
]}

机构：

[1] Nagaoka Univ Technol, Dept Bioengn, Nagaoka, Niigata 94021, Japan

来源：

POLYMER | 1998年 / 39卷 / 11期

关键词：

poly[1-(2-carboxyethyl)pyrrole; glucose oxidase; glucose sensing;

D O I：

10.1016/S0032-3861(97)00531-4

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Poly[1-(2-carboxyethyl)pyrrole] film (PPy-COOH film) was electrochemically prepared and glucose oxidase (GOD) was covalently immobilized on the PPy-COOH him by the condensation reaction with carboxyl groups of the PPy-COOH. The apparent activity of the GOD immobilized on the PPy-COOH film was 152 mU cm(-2). The GOD-immobilized PPy-COOH film (PPy-GOD film) was applied to amperometric glucose sensing. Plots of response current versus glucose concentration gave a straight line with a slope of 1.7 mu A cm(-2) per 1 mM glucose up to 80 mM. The glucose oxidation with the GOD on the PPy-GOD film was considered to proceed through a Michaelis-Menten mechanism. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：2079 / 2082

页数：4

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