Protein-lipid interactions and enzyme requirements for light subtype generation on cycling reconstituted surfactant

被引:7
作者
Dhand, R [1 ]
Sharma, VK
Teng, AL
Krishnasamy, S
Gross, NJ
机构
[1] Edward Hines Jr Vet Affairs Hosp, Div Pulm & Crit Care Med, Hines, IL 60141 USA
[2] Loyola Univ, Stritch Sch Med, Hines, IL 60141 USA
关键词
surfactant; surfactant metabolism; surfactant artificial; surfactant proteins; surfactant convertase;
D O I
10.1006/bbrc.1998.8325
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Surfactant convertase is required for conversion of heavy density (H) natural surfactant to light density (L) subtype during cycling in vitro, a technique that reproduces surfactant metabolism. To study mechanisms of H to L conversion, we prepared liposomes of dipalmitoylphosphatidylcholine (DPPC) and phosphatidylglycerol (PG), or the phospholipids (PL) in combination with either surfactant protein A (SP-A), surfactant protein B (SP-B), or both SP-A and SP-B. Phospholipids alone showed time dependent conversion from heavy to light subtype on cycling in the absence of convertase, which was decreased by adding SP-B, but not SP-A, to phospholipids (p<0.01 for PL+SP-B, or PL+SP-A+SP-B vs. FL, or PL+SP-A). The ultrastructure, surface activity, buoyant density, and L subtype generation on cycling PL+SP-A+SP-B with partially purified convertase or with phospholipase D were similar to those of natural TM. In conclusion, a reconstituted surfactant mimics the behavior of natural surfactant on cycling, and reveals that interaction of SP-B with phospholipids decreases L subtype generation. In addition, esterase/phospholipase D activity is required for conversion of heavy to light subtype on cycling. (C) 1998 Academic Press.
引用
收藏
页码:712 / 719
页数:8
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