Crystal structures of the photosystem II D1 C-terminal processing protease

被引:101
作者
Liao, DI [1 ]
Qian, J [1 ]
Chisholm, DA [1 ]
Jordan, DB [1 ]
Diner, BA [1 ]
机构
[1] DuPont Co Inc, Cent Res & Dev, Expt Stn, Wilmington, DE 19880 USA
关键词
D O I
10.1038/78973
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here the first three-dimensional structure of the D1 C-terminal processing protease (DIP), which is encoded by the ctpA gene. This enzyme removes the C-terminal extension of the D1 polypeptide of photosystem II of oxygenic photosynthesis, Proteolytic processing is necessary to allow the light driven assembly of the tetranuclear manganese duster, which is responsible for photosynthetic water oxidation. The X-ray structure of the Scenedesmus obliquus enzyme has been determined at 1.8 Angstrom resolution using the multiwavelength anomalous dispersion method. The enzyme is monomeric and is composed of three folding domains. The middle domain is topologically homologous to known PDZ motifs and is proposed to be the site at which the substrate C-terminus binds. The remainder of the substrate likely extends across the face of the enzyme, interacting at its scissile bond with the enzyme active site Ser 372/Lys 397 catalytic dyed, which lies at the center of the protein at the interface of the three domains.
引用
收藏
页码:749 / 753
页数:5
相关论文
共 39 条
[1]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[2]   Substrate recognition through a PDZ domain in tail-specific protease [J].
Beebe, KD ;
Shin, JN ;
Peng, J ;
Chaudhury, C ;
Khera, J ;
Pei, DH .
BIOCHEMISTRY, 2000, 39 (11) :3149-3155
[3]   CRYSTALLOGRAPHIC R-FACTOR REFINEMENT BY MOLECULAR-DYNAMICS [J].
BRUNGER, AT ;
KURIYAN, J ;
KARPLUS, M .
SCIENCE, 1987, 235 (4787) :458-460
[4]   Crystal structure of a PDZ domain [J].
Cabral, JHM ;
Petosa, C ;
Sutcliffe, MJ ;
Raza, S ;
Byron, O ;
Poy, F ;
Marfatia, SM ;
Chishti, AH ;
Liddington, RC .
NATURE, 1996, 382 (6592) :649-652
[5]  
*COLL COMP PROJ NU, 1994, CRYSTALLOGRAPHY, V31, P34
[6]  
Cowtan K., 1994, JOINT CCP4 ESF EACBM, V31, P34
[7]   Crystal structures of a complexed and peptide-free membrane protein-binding domain: Molecular basis of peptide recognition by PDZ [J].
Doyle, DA ;
Lee, A ;
Lewis, J ;
Kim, E ;
Sheng, M ;
MacKinnon, R .
CELL, 1996, 85 (07) :1067-1076
[8]   PHASES-95: A program package for processing and analyzing diffraction data from macromolecules [J].
Furey, W ;
Swaminathan, S .
MACROMOLECULAR CRYSTALLOGRAPHY, PT B, 1997, 277 :590-+
[9]   CLONING, MAPPING, AND CHARACTERIZATION OF THE ESCHERICHIA-COLI PRC GENE, WHICH IS INVOLVED IN C-TERMINAL PROCESSING OF PENICILLIN-BINDING PROTEIN-3 [J].
HARA, H ;
YAMAMOTO, Y ;
HIGASHITANI, A ;
SUZUKI, H ;
NISHIMURA, Y .
JOURNAL OF BACTERIOLOGY, 1991, 173 (15) :4799-4813
[10]   THE TREATMENT OF METASTATIC BREAST-CANCER WITH ADJUVANT SYSTEMIC THERAPY [J].
HENDERSON, IC .
ANNALS OF ONCOLOGY, 1990, 1 (01) :9-10