Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs

被引：40

作者：

Luna, VA

King, D

Davis, C

Rycerz, T

Ewert, M

Cannons, A

Amuso, P

Cattani, J

机构：

[1] Univ S Florida, Ctr Dev Biol, Dept Environm & Occupat Hlth, Coll Publ Hlth, Tampa, FL 33612 USA

[2] Florida Dept Hlth, Bur Labs, Tampa, FL 33612 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 03期

关键词：

D O I：

10.1128/JCM.41.3.1252-1255.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.

引用

页码：1252 / 1255

页数：4

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