Biogenic Silver for Disinfection of Water Contaminated with Viruses

被引：104

作者：

De Gusseme, Bart ^{[1
]}

Sintubin, Liesje ^{[1
]}

Baert, Leen ^{[2
]}

Thibo, Ellen ^{[1
]}

Hennebel, Tom ^{[1
]}

Vermeulen, Griet ^{[1
]}

Uyttendaele, Mieke ^{[2
]}

Verstraete, Willy ^{[1
]}

Boon, Nico ^{[1
]}

机构：

[1] Univ Ghent, Lab Microbial Ecol & Technol, Dept Biochem & Microbiol Technol, B-9000 Ghent, Belgium

[2] Univ Ghent, Lab Food Microbiol & Food Preservat, Dept Food Safety & Food Qual, B-9000 Ghent, Belgium

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 04期

关键词：

ESCHERICHIA-COLI; MURINE NOROVIRUS; NANOPARTICLES; STABILITY; NITRATE; MODEL;

D O I：

10.1128/AEM.02433-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of enteric viruses in drinking water is a potential health risk. Growing interest has arisen in nanometals for water disinfection, in particular the use of silver-based nanotechnology. In this study, Lactobacillus fermentum served as a reducing agent and bacterial carrier matrix for zerovalent silver nanoparticles, referred to as biogenic Ag-0. The antiviral action of biogenic Ag-0 was examined in water spiked with an Enterobacter aerogenes-infecting bacteriophage (UZ1). Addition of 5.4 mg liter(-1) biogenic Ag-0 caused a 4.0-log decrease of the phage after 1 h, whereas the use of chemically produced silver nanoparticles (nAg(0)) showed no inactivation within the same time frame. A control experiment with 5.4 mg liter(-1) ionic Ag+ resulted in a similar inactivation after 5 h only. The antiviral properties of biogenic Ag-0 were also demonstrated on the murine norovirus 1 (MNV-1), a model organism for human noroviruses. Biogenic Ag-0 was applied to an electropositive cartridge filter (NanoCeram) to evaluate its capacity for continuous disinfection. Addition of 31.25 mg biogenic Ag-0 m(-2) on the filter (135 mg biogenic Ag-0 kg(-1) filter medium) caused a 3.8-log decline of the virus. In contrast, only a 1.5-log decrease could be obtained with the original filter. This is the first report to demonstrate the antiviral efficacy of extracellular biogenic Ag-0 and its promising opportunities for continuous water disinfection.

引用

页码：1082 / 1087

页数：6

共 44 条

[1]

Adams MH, 1959, BACTERIOPHAGES, DOI 10.5962/bhl.title.6966

[2] Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum [J].

Ahmad, A ;

Mukherjee, P ;

Senapati, S ;

Mandal, D ;

Khan, MI ;

Kumar, R ;

Sastry, M .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2003, 28 (04) :313-318

[3]

[Anonymous], [No title captured], Patent No. 6838005

[4]

[Anonymous], 2004, Water and Sanitation and Hygiene Links to Health: Facts and Figures

[5] Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure [J].

Baert, Leen ;

Wobus, Christiane E. ;

Van Coillie, Els ;

Thackray, Larissa B. ;

Debevere, Johan ;

Uyttendaele, Mieke .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (02) :543-546

[6] Nanoparticle silver released into water from commercially available sock fabrics [J].

Benn, Troy M. ;

Westerhoff, Paul .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (11) :4133-4139

[7] Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus [J].

Birla, S. S. ;

Tiwari, V. V. ;

Gade, A. K. ;

Ingle, A. P. ;

Yadav, A. P. ;

Rai, M. K. .

LETTERS IN APPLIED MICROBIOLOGY, 2009, 48 (02) :173-179

[8] Waterborne outbreak of Norwalk-like virus gastroenteritis at a tourist resort, Italy [J].

Boccia, D ;

Tozzi, AE ;

Cotter, B ;

Rizzo, C ;

Russo, T ;

Buttinelli, G ;

Caprioli, A ;

Marziano, ML ;

Ruggeri, FM .

EMERGING INFECTIOUS DISEASES, 2002, 8 (06) :563-568

[9] Surrogates for the study of norovirus stability and inactivation in the environment:: A comparison of murine norovirus and feline calicivirus [J].

Cannon, Jennifer L. ;

Papafragkou, Efstathia ;

Park, Geunwoo W. ;

Osborne, Jason ;

Jaykus, Lee-Ann ;

Vinje, Jan .

JOURNAL OF FOOD PROTECTION, 2006, 69 (11) :2761-2765

[10] Nitrate-reducing, sulfide-oxidizing bacteria as microbial oxidants for rapid biological sulfide removal [J].

De Gusseme, Bart ;

De Schryver, Peter ;

De Cooman, Michael ;

Verbeken, Kim ;

Boeckx, Pascal ;

Verstraete, Willy ;

Boon, Nico .

FEMS MICROBIOLOGY ECOLOGY, 2009, 67 (01) :151-161

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