A sensitive fluorescence anisotropy method for the direct detection of cancer cells in whole blood based on aptamer-conjugated near-infrared fluorescent nanoparticles

被引：51

作者：

Deng, Ting ^{[1
,2
]}

Li, Jishan ^{[1
]}

Zhang, Liang-Liang ^{[1
]}

Jiang, Jian-Hui ^{[1
]}

Chen, Jie-Nan ^{[2
]}

Shen, Guo-Li ^{[1
]}

Yu, Ru-Qin ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

[2] Cent S Univ Forestry & Technol, Inst Biol & Environm Sci & Technol, Changsha 410004, Hunan, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Core-shell NIR fluorescent nanoparticles; Fluorescence anisotropy; Aptamer; Leukemia cell; Whole blood;

D O I：

10.1016/j.bios.2009.11.014

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Based on the aptamer-conjugated core-shell near-infrared fluorescent nanoparticles (NIR-Nps) and fluorescence anisotropy measurement, the present study reported proof-of-principle for a rapid homogeneous assay approach that can detect target cancer cells without the need of the complicated separation steps in whole blood samples. Experimental investigation showed that the novel NIR-Nps have negligible background fluorescence and low inner filtration interference in complex biologic systems such as whole blood. The specific recognition characteristic of aptamer in whole blood samples was investigated by using the proposed fluorescence anisotropy method. The results showed that the fluorescent nanoparticle-tagged aptamer probes sequence could achieve specific recognition of the target cancer cells from complex mixtures including whole blood samples. And the reaction conditions for the binding between fluorescent nano particle-conjugated aptamer probes and target cancer cells were optimized. The present approach can exhibit sensitive and reproducible fluorescence anisotropy responses to the target cells concentration and the calibration curve showed good linearity when the target cells concentration is in the range from 4.0 x 10(3) to 7.0 x 1(0)5 cells/mL. Moreover, the present fluorescence anisotropy assay technique could be practically utilized for the detection of acute leukemia samples with improved capabilities and be comparable to the immunophenotyping methods clinically used. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1587 / 1591

页数：5

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