Respiratory pathways of Rhodobacter sphaeroides 2.4.1T:: identification and characterization of genes encoding quinol oxidases

被引:16
作者
Mouncey, NJ [1 ]
Gak, E [1 ]
Choudhary, M [1 ]
Oh, JI [1 ]
Kaplan, S [1 ]
机构
[1] Univ Texas, Hlth Sci Ctr, Sch Med, Dept Microbiol & Mol Genet, Houston, TX 77030 USA
关键词
quinol oxidase; genetics; aerobiosis; R; sphaeroides;
D O I
10.1111/j.1574-6968.2000.tb09383.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rhodobacter sphaeroides 2.4.1(T) respires aerobically via a branched respiratory chain consisting of both cytochrome c oxidases and quinol oxidases. Here, genes from chromosome II encoding two distinct quinol oxidases have been characterized. The qoxBA genes encode a putative heme-copper quinol oxidase, whereas the qxtAB genes encode a quinol oxidase homologous to the cyanide-insensitive oxidase of Pseudomonas aeruginosa. No phenotype was observed for mutations in either oxidase in the wild-type background. A strain containing a qxtA mutation in a cytochrome bc(1) complex mutant background was unable to grow aerobically. No role was found for the Qox oxidase, nor was a qoxB::lacZ transcriptional fusion expressed under a variety of conditions. These are the first molecular studies to characterize the quinol oxidases of R. sphaeroides 2.4.1(T). (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:205 / 210
页数:6
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