The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase

被引：62

作者：

De Bie, LGS

Roovers, M

Oudjama, Y

Wattiez, R

Tricot, C

Stalon, V

Droogmans, L

Bujnicki, JM

机构：

[1] Free Univ Brussels, Microbiol Lab, Inst Rech Microbiol JM Wiame, B-1070 Brussels, Belgium

[2] Univ Mons, Chim Biol Lab, B-7000 Mons, Belgium

[3] Int Inst Mol & Cell Biol, Bioinformat Lab, PL-02109 Warsaw, Poland

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 10期

关键词：

D O I：

10.1128/JB.185.10.3238-3243.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N-7-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.

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收藏

页码：3238 / 3243

页数：6

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