Spatiotemporal patterns of Musashil expression during inner ear development

被引：14

作者：

Sakaguchi, H

Yaoi, T

Suzuki, T

Okano, H

Hisa, Y

Fushiki, S ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Pathol & Appl Neurobiol, Kamigyo Ku, Kyoto 6028566, Japan

[2] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Otolaryngol Head & Neck Surg, Kamigyo Ku, Kyoto 6028566, Japan

[3] Keio Univ, Sch Med, Dept Physiol, Shinjuku Ku, Tokyo 1608582, Japan

来源：

NEUROREPORT | 2004年 / 15卷 / 06期

关键词：

cochlea; development; hair cells; immunohistochemistry; inner ear; Musashil; RNA binding protein; RT-PCR; supporting cells; vestibule;

D O I：

10.1097/00001756-200404290-00013

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Musashil (Msi1) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells. To investigate the involvement of Msi1 in the inner ear development, we studied the expression of Msi1 in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that Msi1 was expressed in all otocyst cells at embryonic day (E) 10 and 12. Msi1 immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of Msi1 changed from "cytoplasmic predominance" to "nuclear predominance" during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.

引用

页码：997 / 1001

页数：5

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