Agrobacterium mediated transformation of 'Alpine' Fragaria vesca, and transmission of transgenes to R1 progeny

被引：32

作者：

Haymes, KM ^{[1
]}

Davis, TM ^{[1
]}

机构：

[1] Univ New Hampshire, Dept Plant Biol, Durham, NH 03824 USA

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 04期

关键词：

PCR; progeny-analysis; strawberry; transformation;

D O I：

10.1007/s002990050392

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Agrobacterium-mediated transformation was used to stably introduce beta-glucuronidase (gus) and neomycin phosphotransferase (nptII) marker genes into 'Alpine' Fragaria vesca FRA 197, a diploid (2n = 2x = 14) strawberry. RO generation transformants derived from a single clump of kanamycin-resistant callus were vegetatively propagated. The presence of the gus and nptII genes in five clonal RO runner plants was confirmed by PCR. Southern analysis suggested two sites of nptII insertion. When R1 generation seedlings obtained via self-fertilization of RO plants were tested by histochemical assay, 591 were GUS positive and 39 were GUS negative. The R1 segregation data fit a 15 : 1 ratio (0.5>P>0.25), consistent with the independent segregation of two transgene insertion loci. These results demonstrate the suitability of 'Alpine' F. vesca for transgene research in strawberry.

引用

页码：279 / 283

页数：5

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