Galactosylated chitosan-graft-dextran as hepatocyte-targeting DNA carrier

被引：130

作者：

Park, YK

Park, YH

Shin, BA

Choi, ES

Park, YR

Akaike, T

Cho, CS ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Agr Biotechnol, Suwon 441744, South Korea

[2] Seoul Natl Univ, Sch Biol Resources & Mat Engn, Suwon 441744, South Korea

[3] Chonnam Univ, Sch Med, Inst Med Sci, Kwangju 501190, South Korea

[4] Tokyo Inst Technol, Fac Biosci & Biotechnol, Yokohama, Kanagawa 227, Japan

来源：

JOURNAL OF CONTROLLED RELEASE | 2000年 / 69卷 / 01期

关键词：

galactosylated chitosan; liver-specific; DNA carrier; asialoglycoprotein receptor;

D O I：

10.1016/S0168-3659(00)00298-4

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Lactobionic acid bearing galactose group was coupled with chitosan for liver specificity, and dextran was grafted to galactosylated chitosan (GC) for stability in water. Compared to the GC/DNA complex, the stability of the galactosylated chitosan-graft-dextran (GCD)/DNA complex could be enhanced. The particle size of the GCD/DNA complexes decreased as the charge ratio of GCD to DNA increased. Conformational change of DNA did not occur after complex formation with GCD compared with the conformation of DNA itself. The GCD/DNA complexes were only transfected into Chang liver cells and that of Hep G2 having asialoglycoprotein receptors (ASGR), indicative of specific interaction of ASGRs on cells and galactose ligands on chitosan. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：97 / 108

页数：12

共 23 条

[1] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].