Production and characterization of murine single chain Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system

被引：31

作者：

Daly, SJ ^{[1
]}

Dillon, PP

Manning, BM

Dunne, L

Killard, A

O'Kennedy, R

机构：

[1] Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland

[2] Dublin City Univ, Natl Ctr Sensor Res, Dublin 9, Ireland

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2002年 / 14卷 / 04期

关键词：

aflatoxin B-1; single chain Fv; phage display technology; surface plasmon resonance;

D O I：

10.1080/0954010021000096373

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The production and characterization of recombinant antibodies to aflatoxin B-1 (AFB(1) ), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B-1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB(1) , in this format, with the assays being sensitive and reproducible.

引用

收藏

页码：255 / 274

页数：20

相关论文

共 30 条

[1] Use of phosphonic acid as a generic hapten in the production of broad specificity anti-organophosphate pesticide antibody [J].

Alcocer, MJC ;

Dillon, PP ;

Manning, BM ;

Doyen, C ;

Lee, HA ;

Daly, SJ ;

O'Kennedy, R ;

Morgan, MRA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (06) :2228-2233

[2] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[3] Immunoassay for the determination of morphine-3-glucuronide using a surface plasmon resonance-based biosensor [J].

Dillon, PP ;

Daly, SJ ;

Manning, BM ;

O'Kennedy, R .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (2-3) :217-227

[4] Validation of immunoassays for bioanalysis: a pharmaceutical industry perspective [J].

Findlay, JWA ;

Smith, WC ;

Lee, JW ;

Nordblom, GD ;

Das, I ;

DeSilva, BS ;

Khan, MN ;

Bowsher, RR .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2000, 21 (06) :1249-1273

[5]

HERMANSON GT, 1996, BIOCONJUGATE TECHNIQ, P420

[6] RAPID AND EFFICIENT PURIFICATION OF NATIVE HISTIDINE-TAGGED PROTEIN EXPRESSED BY RECOMBINANT VACCINIA VIRUS [J].

JANKNECHT, R ;

DEMARTYNOFF, G ;

LOU, J ;

HIPSKIND, RA ;

NORDHEIM, A ;

STUNNENBERG, HG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) :8972-8976

[7]

Kabat E. A., 1991, SEQUENCES PROTEINS I

[8] OPTIMIZATION OF PRIMERS FOR CLONING LIBRARIES OF MOUSE IMMUNOGLOBULIN GENES USING THE POLYMERASE CHAIN-REACTION [J].

KETTLEBOROUGH, CA ;

SALDANHA, J ;

ANSELL, KH ;

BENDIG, MM .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (01) :206-211

[9] Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system [J].

Krebber, A ;

Bornhauser, S ;

Burmester, J ;

Honegger, A ;

Willuda, J ;

Bosshard, HR ;

Pluckthun, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 201 (01) :35-55

[10] BIA/MS: Interfacing biomolecular interaction analysis with mass spectrometry [J].

Krone, JR ;

Nelson, RW ;

Dogruel, D ;

Williams, P ;

Granzow, R .

ANALYTICAL BIOCHEMISTRY, 1997, 244 (01) :124-132