Conserved methionines in chloroplasts

被引:33
作者
Sundby, C
Härndahl, U
Gustavsson, N
Åhrman, E
Murphy, DJ
机构
[1] Lund Univ, Dept Biochem, Ctr Chem & Chem Engn, S-22100 Lund, Sweden
[2] Biovitrum AB, S-11276 Stockholm, Sweden
[3] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
[4] Univ Glamorgan, Biotechnol Unit, Sch Appl Sci, Pontypridd CF37 1DL, M Glam, Wales
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1703卷 / 02期
关键词
chaperone; heat stress; oxidative stress; photosynthesis; protein mass spectrometry; protein protein interaction;
D O I
10.1016/j.bbapap.2004.09.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Heat shock proteins counteract heat and oxidative stress. In chloroplasts, a small heat shock protein (Hsp21) contains a set of conserved methionines, which date back to early in the emergence of terrestrial plants. Methionines M49, M52, M55, M59, M62, M67 are located on one side of an amphipathic helix, which may fold back over two other conserved methionines (M97 and M101), to form a binding groove lined with methionines, for sequence-independent recognition of peptides with an overall hydrophobic character. The sHsps protect other proteins from aggregation by binding to their hydrophobic surfaces, which become exposed under stress. Data are presented showing that keeping the conserved methionines in Hsp21 in a reduced form is a prerequisite to maintain such binding. The chloroplast generates reactive oxygen species under both stress and unstressed conditions, but this organelle is also a highly reducing cellular compartment. Chloroplasts contain a specialized isoform of the enzyme, peptide methionine sulfoxide reductase, the expression of which is light-induced. Recombinant proteins were used to measure that this reductase can restore Hsp21 methionines after sulfoxidation. This paper also describes how methionine sulfoxidation-reduction can be directly assessed by mass spectrometry, how methionine-to-leucine substitution affects Hsp21, and discusses the possible role for an Hsp21 methionine sulfoxidation-reduction cycle in quenching reactive oxygen species. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:191 / 202
页数:12
相关论文
共 67 条
[1]   Reversible methionine sulfoxidation of Mycobacterium tuberculosis small heat shock protein Hsp16.3 and its possible role in scavenging oxidants [J].
Abulimiti, A ;
Qiu, XL ;
Chen, J ;
Liu, Y ;
Chang, ZY .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 305 (01) :87-93
[2]   Dual regulation of a heat shock promoter during embryogenesis: stage-dependent role of heat shock elements [J].
Almoguera, C ;
Prieto-Dapena, P ;
Jordano, J .
PLANT JOURNAL, 1998, 13 (04) :437-446
[3]  
Arrigo AP, 1998, BIOL CHEM, V379, P19
[4]  
ARRIGO AP, 1991, HEAT SHOCK DEV, P106
[5]   Chloroplast proteomics: potentials and challenges [J].
Baginsky, S ;
Gruissem, W .
JOURNAL OF EXPERIMENTAL BOTANY, 2004, 55 (400) :1213-1220
[6]   The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions [J].
Basha, E ;
Lee, GJ ;
Breci, LA ;
Hausrath, AC ;
Buan, NR ;
Giese, KC ;
Vierling, E .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (09) :7566-7575
[7]   Arabidopsis peptide methionine sulfoxide reductase2 prevents cellular oxidative damage in long nights [J].
Bechtold, U ;
Murphy, DJ ;
Mullineaux, PM .
PLANT CELL, 2004, 16 (04) :908-919
[8]   Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acetyltransferase [J].
Blaszczyk, A ;
Brodzik, R ;
Sirko, A .
PLANT JOURNAL, 1999, 20 (02) :237-243
[9]   Genealogy of the α-crystallin -: small heat-shock protein superfamily [J].
de Jong, WW ;
Caspers, GJ ;
Leunissen, JAM .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1998, 22 (3-4) :151-162
[10]   Sulfur assimilation and the role of sulfur in plant metabolism: a survey [J].
Droux, M .
PHOTOSYNTHESIS RESEARCH, 2004, 79 (03) :331-348