Solid-phase extraction for multiresidue determination of sulfonamides, tetracyclines, and pyrimethamine in bovine's milk

被引：85

作者：

Koesukwiwat, Urairat ^{[1
]}

Jayanta, Siripastr ^{[1
]}

Leepipatpiboon, Natchanun ^{[1
]}

机构：

[1] Chulalongkorn Univ, Dept Chem, Fac Sci, Chromatog & Separat Res Grp, Bangkok 10330, Thailand

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1149卷 / 01期

关键词：

sulfonamide; tetracycline; pyrimethamine; solid-phase extraction; antibiotics; milk;

D O I：

10.1016/j.chroma.2007.02.075

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a systematic approach to the development of a solid-phase extraction method for simultaneous extraction of 10 antibiotic residues in bovine milk, belonging to groups of sulfonamides, tetracyclines, and pyrimethamine. The sample preparation steps include acidic deproteinization of milk proteins followed by sample enrichment and cleanup using a polymer-based Oasis HLB solid-phase extraction cartridge. The analyses were carried out by using a method based on liquid chromatography-electrospray ionisation-mass spectrometry with positive ion mode. The parameters affecting the extraction efficiency such as sample loading pH, SPE wash solvent composition, and eluting solution pH were carefully investigated and optimized. The developed solid-phase extraction procedure coupled to multiresidue liquid chromatography-electrospray ionization-mass spectrometry method was applied for the analysis of 10 antibiotic residues in milk samples, and it proved to be simple, sensitive, and selective providing a recovery ranging from 70 to 106%. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：102 / 111

页数：10

共 31 条

[1]

*AGR FOOD PHARM FO, 2003, OASIS APPL NOT

[2] Determination of tetracycline residues in shrimp and whole milk using liquid chromatography with ultraviolet detection and residue confirmation by mass spectrometry [J].