Structure of a ribonuclease B+d(pA)(4) complex

被引:5
作者
Ko, TP
Williams, R
McPherson, A
机构
[1] Department of Biochemistry, University of California, Riverside
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1996年 / 52卷
关键词
D O I
10.1107/S0907444995009127
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 Angstrom. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any poly saccharide residues or solvent molecules. After refinement at 2.7 Angstrom, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.
引用
收藏
页码:160 / 164
页数:5
相关论文
共 22 条